the correlation between telomerase activity and bax/bcl-2 ratio in valproic acid-treated mcf-7 breast cancer cell line
نویسندگان
چکیده
objective(s):valproic acid (vpa), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. a relation between telomerase activity and resistance to apoptosis has been established. this study focused on probable effects of vpa on mcf-7 cancer cells. in particular, we investigated vpa effects on viability, apoptosis and telomerase activity. materials and methods: cytotoxicity effects of vpa on mcf-7 cells were determined by neutral red uptake assay. cells were treated with different concentrations of vpa (0-32 mm) and telomerase activity and bax and bcl-2 protein levels were determined using trap assay (pcr-elisa) method and elisa method, respectively. results: the cytotoxic effects of different concentration of vpa on mcf-7 cells were observed as a reduction in cell viability and telomerase activity and altered expression of bcl-2 family protein levels. the results also showed that there is a significant correlation between reduction of telomerase activity and increase in bax/bcl-2 ratio (p=0.001). conclusion: our study demonstrated that cell viability of mcf-7 cells was decreased after treatment with vpa, probably through a reduction of telomerase activity and an increase in bax/bcl-2 ratio. therefore, it could be concluded that vpa is a potent anti-cancer agent for breast cancer cells through inhibition of telomerase activity and induction of apoptosis.
منابع مشابه
The correlation between telomerase activity and Bax/Bcl-2 ratio in valproic acid-treated MCF-7 breast cancer cell line
Objective(s):Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. T...
متن کاملThe correlation between telomerase activity and Bax/Bcl-2 ratio in valproic acid-treated MCF-7 breast cancer cell line
OBJECTIVES Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. Thi...
متن کاملthe simultaneous effect of valproic acid and gamma radiation on telomerase activity and bax and bcl-2 protein levels in mcf-7 breast cancer cell line
conclusions the increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the valproic acid and radiation mechanism. the current survey suggested that it is likely beneficial to combine valproic acid and gamma radiation to treat breast cancer. results combination of valproic acid and gamma radiation increased significantly cell toxicit...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۸، شماره ۷، صفحات ۷۰۰-۷۰۴
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